2DLB
X-ray Crystal Structure of Protein yopT from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR412
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9787, 0.9794, 0.9678 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.347, 76.116, 78.385 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.200 |
| R-free | 0.17300 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.031 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 53730 | |
| Completeness [%] | 87.5 | 59.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 0.1M lithium sulfate, 0.1M sodium citrate, 20% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 4.0 |
