2DLA

Primase large subunit amino terminal domain from Pyrococcus horikoshii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-07
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	89.250, 89.250, 252.674
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.900
Rwork	0.248
R-free	0.30940
Structure solution method	MAD, MOLECULAR REPLACEMENT
Starting model (for MR)	seleno-methionie derivative structure(This is not deposited to PDB)
RMSD bond length	0.008
RMSD bond angle	1.364
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.930
High resolution limit [Å]	2.900	2.900
Rmerge	0.044	0.218
Number of reflections	23431
<I/σ(I)>	17	7.3
Completeness [%]	99.2	100
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1M Ammonium Sulfate, 50mM Magnesium Sulfate, 25mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1M Ammonium Sulfate, 50mM Magnesium Sulfate, 25mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1M Ammonium Sulfate, 50mM Magnesium Sulfate, 25mM Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K