2DKV
Crystal structure of class I chitinase from Oryza sativa L. japonica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2004-12-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 66.452, 66.452, 123.636 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.220 - 2.000 |
R-factor | 0.17717 |
Rwork | 0.175 |
R-free | 0.22345 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.568 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.220 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.050 | 0.238 |
Number of reflections | 19363 | |
<I/σ(I)> | 10.1 | 3.2 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 13.7 | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 8%(w/v) PEG 20000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |