Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 89.800, 58.800, 102.000 |
Unit cell angles | 90.00, 94.80, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.18 * |
Rwork | 0.185 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dik |
RMSD bond length | 0.018 |
RMSD bond angle | 2.790 |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.660 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.099 * | |
Total number of observations | 97563 * | |
Number of reflections | 35455 | |
Completeness [%] | 95.0 | 83 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 * | 30 * | pH 7.0, temperature 303K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 50-55 (%sat) | |
2 | 1 | reservoir | HEPES | 100 (mM) | |
3 | 1 | drop | protein | 10 (mg/ml) | |
4 | 1 | drop | imidazole | 20 (mM) | |
5 | 1 | drop | 100 (mM) | ||
6 | 1 | drop | EDTA | 0.1 (mM) | |
7 | 1 | drop | mercaptoethanol | 1 (mM) |