2DI4

Crystal structure of the FtsH protease domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-04
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00000, 1.05000, 1.00524, 1.00714
Spacegroup name	P 63
Unit cell lengths	116.800, 116.800, 63.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.790
R-factor	0.2544
Rwork	0.251
R-free	0.29903
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.360
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.890
High resolution limit [Å]	2.790	2.790
Number of reflections	12436
<I/σ(I)>	42.9	9.9
Completeness [%]	99.3	93.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1M MES (pH 6.0), 5% PEG8K, VAPOR DIFFUSION, HANGING DROP, temperature 293K