2DH5
Crystal structure of E. coli Holo-TrpB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-26 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 172.516, 172.516, 82.587 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.330 - 2.900 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | CHAIN B of 1BKS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.400 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.076 | 0.390 |
Number of reflections | 15920 | |
Completeness [%] | 96.8 | 97.4 |
Redundancy | 4.7 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | micro batch using paraffin oil | 8 | 288 | ammonium sulfate, pH 8.0, micro batch using paraffin oil, temperature 288K |