2DH1
Crystal structure of peanut lectin lactose-azobenzene-4,4'-dicarboxylic acid-lactose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 |
| Unit cell lengths | 92.750, 92.750, 473.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 7.650 |
| R-factor | 0.35562 |
| Rwork | 0.355 |
| R-free | 0.37720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pel |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 7.800 |
| High resolution limit [Å] | 7.650 | 7.650 |
| Rmerge | 0.100 | 0.490 |
| Number of reflections | 2273 | |
| <I/σ(I)> | 4.6 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.1 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 12% PEG 8000, 0.05M sodium phosphate, 0.2M sodium cholride, 0.02% sodium azide, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
