2DGM
Crystal structure of Escherichia coli GadB in complex with iodide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2003-05-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.300 |
| Spacegroup name | P 1 |
| Unit cell lengths | 91.336, 91.804, 93.914 |
| Unit cell angles | 76.65, 76.94, 78.04 |
Refinement procedure
| Resolution | 40.000 - 1.950 |
| Rwork | 0.227 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pmm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.260 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 191280 | |
| <I/σ(I)> | 5.9 | 2.7 |
| Completeness [%] | 92.7 | 84.4 |
| Redundancy | 1.8 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.135M sodium acetate, 0.7M sodium formate, 13% PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
