2DGD
Crystal structure of ST0656, a function unknown protein from Sulfolobus tokodaii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9792, 0.9795, 0.9685 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 131.221, 131.221, 125.896 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.990 - 2.900 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.22200 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 31282 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 9.3 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES, 0.6M NaCl, 20% PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
