2DGC
GCN4 BASIC DOMAIN, LEUCINE ZIPPER COMPLEXED WITH ATF/CREB SITE DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.660, 58.660, 86.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.200 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.31600 |
| Data reduction software | OSCREF |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.090 |
| Number of reflections | 6597 |
| Completeness [%] | 80.8 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.6 | pH 4.60, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 6000 | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | NA CITRATE | ||
| 5 | 1 | 1 | NA AZIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | protein DNA complex | 0.2 (mM) | ||
| 3 | 1 | 2 | PEG6000 | 10 (%) | |
| 4 | 1 | 2 | sodium citrate | 30 (mM) | pH4.6 |
| 5 | 1 | 2 | sodium acetate | 50 (mM) | |
| 6 | 1 | 2 | 5 (mM) | ||
| 7 | 1 | 2 | 1 (mM) |
