2DG8

Crystal structure of the putative trasncriptional regulator SCO7518 from Streptomyces coelicolor A3(2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-12-10
Detector	RIGAKU RAXIS VII
Wavelength(s)	2.29
Spacegroup name	P 1 21 1
Unit cell lengths	51.336, 101.262, 76.914
Unit cell angles	90.00, 98.60, 90.00

Refinement procedure

Resolution	19.790 - 2.210
R-factor	0.218
Rwork	0.218
R-free	0.24700
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.290
High resolution limit [Å]	2.210	2.210
Number of reflections	38773
Completeness [%]	99.7	99.1
Redundancy	14	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	300mM diammonium hydrogen phosphate, 20% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K