2DG7

Crystal structure of the putative transcriptional regulator SCO0337 from Streptomyces coelicolor A3(2)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934, 0.97950, 0.98338
Spacegroup name	P 21 21 2
Unit cell lengths	102.952, 42.864, 43.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.980 - 2.300
R-factor	0.23
Rwork	0.230
R-free	0.27600
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.900
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	9138
Completeness [%]	99.7	97.9
Redundancy	10.2	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100mM HEPES pH7.0, 9% Jeffamine ED-2001 reagent pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K