2DG6
Crystal structure of the putative transcriptional regulator SCO5550 from Streptomyces coelicolor A3(2)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9788, 0.9795, 0.900 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 49.689, 100.442, 38.989 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.200 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.27600 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 11793 | |
| Completeness [%] | 98.3 | 88.8 |
| Redundancy | 6.9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM Bis-Tris pH6.5, 1250mM Ammonium sulfate, 150mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
