2DFL
Crystal structure of left-handed RadA filament
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 |
Unit cell lengths | 55.183, 55.183, 125.576 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.900 |
R-factor | 0.226 |
Rwork | 0.217 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t4g |
RMSD bond length | 0.011 |
RMSD bond angle | 1.800 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.980 |
High resolution limit [Å] | 2.880 | 2.880 |
Rmerge | 0.053 | 0.723 |
Number of reflections | 8521 | |
<I/σ(I)> | 27.8 | 2.1 |
Completeness [%] | 99.8 | 100 |
Redundancy | 8.3 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 3% PEG4000, 50mM Na-acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 3% PEG4000, 50mM Na-acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |