2DFH
Crystal structure of Tk-RNase HII(1-212)-C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.798, 78.782, 46.519 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.120 - 2.270 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1io2 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.350 |
High resolution limit [Å] | 2.270 | 2.270 |
Number of reflections | 9466 | |
Completeness [%] | 98.9 | 91.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 10% PEG 8000, 0.2M MES, 20% glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |