2DFC
Xylanase II from Tricoderma reesei at 293K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2004-11-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.15 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.550, 60.030, 70.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.190 |
| R-factor | 0.106 |
| Rwork | 0.106 |
| R-free | 0.12200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1enx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.029 |
| Data reduction software | TRUNCATE |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.260 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.046 | 0.392 |
| Number of reflections | 67093 | |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 6.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 0.4M ammonium sulfate, 0.1M bicine, 0.15M sodium iodide, 1.6% protein, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
