2DF8
Crystal Structure of the PH0510 protein from Pyrococcus horikoshii OT3 in complex with beta-D-Fructopyranose-1-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-16 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.179, 79.644, 67.494 |
| Unit cell angles | 90.00, 105.24, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dec |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.143 | 0.379 |
| Number of reflections | 99585 | |
| <I/σ(I)> | 5.4 | 2.76 |
| Completeness [%] | 99.0 | 93.6 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.8 | 295 | PEG 3350, lithium acetate, pH 7.8, microbatch, temperature 295K |
