2DEP
Crystal Structure of xylanase B from Clostridium stercorarium F9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-23 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.760, 96.600, 138.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.140 - 1.800 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hiz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.140 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.062 | 0.276 |
| Number of reflections | 80942 | |
| <I/σ(I)> | 8.8 | 2.5 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 24% PEG 1500, 20% glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
