2DEG
Crystal structure of tt0972 protein form Thermus Thermophilus with Mn2(+) ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-19 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 65.324, 65.324, 202.366 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.23845 |
| Rwork | 0.236 |
| R-free | 0.27870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cz8 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.542 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.044 | 0.399 |
| Number of reflections | 49302 | |
| <I/σ(I)> | 33.1 | 4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 30% MPD, 200mM ammonium acetate, 100mM lithium chloride, 10mM manganese chloride, 100mM citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
