2DEC
Crystal Structure of the PH0510 protein from Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-16 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.800 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.228, 79.485, 67.497 |
Unit cell angles | 90.00, 105.15, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.700 |
R-factor | 0.166 |
Rwork | 0.166 |
R-free | 0.18900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j5x |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.091 | 0.344 |
Number of reflections | 68213 | |
<I/σ(I)> | 6.9 | 2.81 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 4.4 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.8 | 295 | PEG 3350, lithium acetate, pH 7.8, microbatch, temperature 295K |