2DEB
Crystal structure of rat carnitine palmitoyltransferase 2 in space group C2221
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97899 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.180, 97.290, 310.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.000 - 1.600 |
| R-factor | 0.16655 |
| Rwork | 0.165 |
| R-free | 0.19375 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.227 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.062 | 0.212 |
| Number of reflections | 188487 | |
| <I/σ(I)> | 14.04 | 7.24 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0.15M DL-malic acid 20% (w/v) PEG 3350 , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
