2DE9

Crystal structure of porcine pancreatic elastase complexed with Tris after soaking a tris-free solution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.8
Spacegroup name	P 21 21 21
Unit cell lengths	49.877, 57.847, 74.329
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.300
Rwork	0.211
R-free	0.22600
Structure solution method	FOURIER SYNTHESIS
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNX
Refinement software	CNX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.300
Number of reflections	52552
Completeness [%]	96.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.3M sodium chloride, 50mM Tris , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K