2DE5

Crystal structure of the electron transfer complex between oxygenase and ferredoxin in carbazole 1,9a-dioxygenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-06
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	98.091, 89.835, 104.914
Unit cell angles	90.00, 103.79, 90.00

Refinement procedure

Resolution	44.920 - 1.900
R-factor	0.199
Rwork	0.199
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vck 1ww9
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.920	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	138880
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1-2mM sodium dithionite, 14% PEG MME 2000, 0.05M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K