2DDK
Crystal structure of human myo-inositol monophosphatase 2 (IMPA2) (orthorhombic form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-27 |
| Detector | ADSC QUAMTUM 4r |
| Wavelength(s) | 0.9780 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.457, 95.639, 107.239 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.820 - 2.700 |
| Rwork | 0.260 |
| R-free | 0.30100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2czh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 19030 | |
| <I/σ(I)> | 28.2 | 2.7 |
| Completeness [%] | 99.2 | 93 |
| Redundancy | 4.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.025M potassium dihydrogen phosphate, 14% PEG8000, 5mM LiCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
