2DDD
Unique behavior of a histidine responsible for an engineered green-to-red photoconversion process
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2003-12-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.837, 118.410, 48.856 |
| Unit cell angles | 90.00, 119.96, 90.00 |
Refinement procedure
| Resolution | 100.000 - 1.550 |
| Rwork | 0.175 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xss |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.620 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 69780 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 99.7 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 4M NaCl, 0.1M HEPES, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
