2DDC
Unique behavior of a histidine responsible for an engineered green-to-red photoconversion process
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2003-11-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.7 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 97.629, 119.286, 49.068 |
Unit cell angles | 90.00, 120.18, 90.00 |
Refinement procedure
Resolution | 100.000 - 1.550 |
Rwork | 0.190 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xss |
RMSD bond length | 0.011 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.620 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 74065 | |
<I/σ(I)> | 6.8 | |
Completeness [%] | 97.9 | 84.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 4M NaCl, 0.1M HEPES, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |