2DCY
Crystal structure of Bacillus subtilis family-11 xylanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 130.445, 186.209, 37.979 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.400 |
R-factor | 0.19828 |
Rwork | 0.197 |
R-free | 0.21733 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xnb |
RMSD bond length | 0.016 |
RMSD bond angle | 1.701 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.044 | 0.301 |
Number of reflections | 178663 | |
<I/σ(I)> | 13.7 | 2.5 |
Completeness [%] | 97.6 | 87.3 |
Redundancy | 3.7 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 0.1M Imidazole, 1.0-1.1K/Na tartrate, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |