2DCK
A tetragonal-lattice structure of alkaliphilic XynJ from Bacillus sp. 41M-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-24 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1 |
Spacegroup name | P 43 |
Unit cell lengths | 116.579, 116.579, 45.909 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.16812 |
Rwork | 0.167 |
R-free | 0.19799 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dcj |
RMSD bond length | 0.026 |
RMSD bond angle | 1.985 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 119.520 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 36572 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 10mM sodium phosphate buffer (Na2HPO4/NaH2PO4), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |