2DCH

Crystal structure of archaeal intron-encoded homing endonuclease I-Tsp061I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-05-13
Detector	RIGAKU RAXIS VII
Spacegroup name	H 3 2
Unit cell lengths	91.593, 91.593, 193.643
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	72.550 - 2.060
R-factor	0.20372
Rwork	0.202
R-free	0.24259
Structure solution method	MIR
RMSD bond length	0.018
RMSD bond angle	1.506
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.550	2.120
High resolution limit [Å]	2.060	2.050
Number of reflections	19290
<I/σ(I)>	36.9	5.6
Completeness [%]	97.8	99.9
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	293	2.2M ammonium sulfate, 0.05M sodium citrate, pH 5.5, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	5.5	293	2.2M ammonium sulfate, 0.05M sodium citrate, pH 5.5, VAPOR DIFFUSION, temperature 293K