2DC5
Crystal structure of mouse glutathione S-transferase, mu7 (GSTM7) at 1.6 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-03 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.385, 94.610, 57.761 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.300 - 1.600 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gtu |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 113517 | |
| <I/σ(I)> | 22.0419 | 7.90167 |
| Completeness [%] | 98.8 | 99.7 |
| Redundancy | 4.62576 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M Sodium Chloride, 0.1M Na HEPES pH 7.5, 28% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
