2DBU
Crystal Structure of Gamma-glutamyltranspeptidase from Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97902, 0.97931, 0.98214 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.700, 126.900, 128.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.390 - 1.950 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.23100 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.059 | 0.369 |
| Number of reflections | 94722 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.2 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 15% PEG 4000, 0.2M magnesium sulfate, 5% glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
