2DBS
Crystal structure of a hypothetical protein TTHC002 from Thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-16 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97924, 0.97957, 0.96000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 41.145, 41.145, 87.572 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.000 - 2.100 |
| Rwork | 0.256 |
| R-free | 0.27120 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.997 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 10858 | |
| <I/σ(I)> | 13.1 | 3.05 |
| Completeness [%] | 96.8 | 80.7 |
| Redundancy | 4.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES-Na, 1.25M Lithium Sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
