2DBO
Crystal structure of D-Tyr-tRNA(Tyr) deacylase from Aquifex aeolicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 76.010, 76.010, 133.754 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.010 - 2.760 |
Rwork | 0.230 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j7g |
RMSD bond length | 0.013 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.010 | 2.860 |
High resolution limit [Å] | 2.760 | 2.760 |
Number of reflections | 6373 | |
<I/σ(I)> | 28.8534 | 7.72 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.3 | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 8 | 277 | 0.15M sodium chloride, pH 8.0, SMALL TUBES, temperature 277K |