2DBI

Crystal Structure of a Hypothetical Protein JW0805 from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-20
Detector	RIGAKU JUPITER 210
Wavelength(s)	1
Spacegroup name	P 31 2 1
Unit cell lengths	69.973, 69.973, 174.804
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.050
R-factor	0.18
Rwork	0.180
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dbn
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	32097
Completeness [%]	93.8	36.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1M bis-tris pH5.5, 25% PEG 3350, 0.2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K