2DBE
CRYSTAL STRUCTURE OF A BERENIL-DODECANUCLEOTIDE COMPLEX: THE ROLE OF WATER IN SEQUENCE-SPECIFIC LIGAND BINDING
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | AREA DETECTOR |
| Detector | XENTRONICS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.510, 39.980, 66.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.500 |
| R-factor | 0.167 |
| Refinement software | CORELS |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.037 |
| Total number of observations | 5762 * |
| Number of reflections | 2195 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 278 | pH 7.00, VAPOR DIFFUSION, temperature 278.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 30 (mM) | ||
| 2 | 1 | drop | berenil | 2 (mM) | |
| 3 | 1 | drop | MPD | 20 (%(v/v)) | |
| 4 | 1 | drop | DNA dodecamer | 3 (mM) | |
| 5 | 1 | drop | sodium cacodylate | 10 (mM) | |
| 6 | 1 | reservoir | 50 (%(v/v)) |
