2DAP
C. GLUTAMICUM DAP DEHYDROGENASE IN COMPLEX WITH DAP
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 290 |
Detector technology | AREA DETECTOR |
Collection date | 1996-01 |
Detector | SIEMENS |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 121.640, 121.640, 52.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
R-factor | 0.192 * |
Rwork | 0.170 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dap |
RMSD bond length | 0.011 |
RMSD bond angle | 26.600 * |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.102 * | 0.293 * |
Total number of observations | 86282 * | |
Number of reflections | 21456 | 2297 * |
<I/σ(I)> | 10.7 | 2.1 |
Completeness [%] | 94.8 | 82.3 |
Redundancy | 4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 13-17% PEG 8000 IN 100 MM NA-CACODYLATE, PH 6.5, 150-300 MM MG-ACETATE CRYSTAL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 12-14 (%) | |
2 | 1 | reservoir | magnesium acetate | 150-300 (mM) | |
3 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
4 | 1 | drop | PEG8000 | 6-7 (%) | |
5 | 1 | drop | magnesium acetate | 75-150 (mM) | |
6 | 1 | drop | sodium cacodylate | 50 (mM) | |
7 | 1 | drop | protein | 10 (mg/ml) | |
8 | 1 | drop | NADP+ | 1 (mM) | |
9 | 1 | drop | DAP | 15 (mM) |