2DAB
L201A MUTANT OF D-AMINO ACID AMINOTRANSFERASE COMPLEXED WITH PYRIDOXAL-5'-PHOSPHATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05-27 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.510, 91.630, 88.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1daa |
| RMSD bond length | 0.009 |
| RMSD bond angle | 23.800 * |
| Data reduction software | PROCESS |
| Data scaling software | PROCESS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.630 | 1.900 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.069 | 0.308 |
| Number of reflections | 48809 | |
| <I/σ(I)> | 9.09 | 1.57 |
| Completeness [%] | 81.0 | 48.3 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 10 | 1 | reservoir | sodium azide | 5 (mM) | |
| 2 | 1 | drop | potassium phosphate | 50 (mM) | |
| 3 | 1 | drop | PLP | 0.05 (M) | |
| 4 | 1 | drop | potassium chloride | 130 (mM) | |
| 5 | 1 | drop | EDTA | 0.2 (M) | |
| 6 | 1 | drop | 2-mercaptoethanol | 0.02 (%) | |
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 8 | 1 | reservoir | PEG3350 | 29-33 (%(w/v)) | |
| 9 | 1 | reservoir | sodium acetate | 300-700 (mM) |
