2D98

Structure of VIL (extra KI/I2 added)-xylanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-08-03
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	40.191, 38.564, 56.794
Unit cell angles	90.00, 110.18, 90.00

Refinement procedure

Resolution	20.100 - 2.000
R-factor	0.209
Rwork	0.209
R-free	0.25300
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.300	2.130
High resolution limit [Å]	2.000	2.000
Number of reflections	10211
Completeness [%]	91.1	80.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.3	293	1.5M ammonium sulfate, 0.1M Na citrate , pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K