2D97
Structure of VIL-xylanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.347, 38.585, 57.162 |
| Unit cell angles | 90.00, 110.31, 90.00 |
Refinement procedure
| Resolution | 18.980 - 2.010 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.22800 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.980 | 2.130 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Number of reflections | 11242 | |
| Completeness [%] | 99.2 | 88.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 293 | 1.5M ammonium sulfate, 0.1M Na citrate, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
