2D94
THE CONFORMATION OF THE DNA DOUBLE HELIX IN THE CRYSTAL IS DEPENDENT ON ITS ENVIRONMENT
Experimental procedure
| Detector technology | CCD |
| Detector | NONIUS CAD4 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 43.280, 43.280, 24.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 1.700 |
| R-factor | 0.162 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 2027 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 * | 292 | pH 7.00, VAPOR DIFFUSION, temperature 292.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 1 | NA CACODYLATE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | DNA | 2.8 (mM) | ||
| 3 | 1 | spermine-4HCl | 0.1 (mM) | ||
| 4 | 1 | MPD | 20 (%) | ||
| 5 | 1 | sodium cacodylate | 50 (mM) | ||
| 6 | 1 | MPD | 40 (%) | pH6.5 |
