2D91
Structure of HYPER-VIL-lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54182 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.692, 79.692, 36.118 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.100 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.29000 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.344 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 7183 | |
| Completeness [%] | 99.8 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 293 | 2.0M NaCl, 0.1M Na acetate, 30% (v/v) glycerol, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
