2D8P
Structure of HYPER-VIL-thaumatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54182 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.727, 57.727, 150.955 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.700 - 2.300 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.25000 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.916 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 21518 | |
| Completeness [%] | 99.8 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.8M K/Na tartrate, 0.1M Na HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
