2D8N
Crystal structure of human recoverin at 2.2 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-03 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.9790, 0.9794, 0.964 |
Spacegroup name | I 4 |
Unit cell lengths | 85.034, 85.034, 65.689 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.520 - 2.200 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.23700 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.800 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 11968 | |
<I/σ(I)> | 23.4162 | 9.35583 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 7.27097 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES-Na pH7.5, 1.2M tri-Sodium Citrate dihydrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |