2D80
Crystal structure of PHB depolymerase from Penicillium funiculosum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2004-11-04 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.507, 68.876, 38.733 |
Unit cell angles | 90.00, 107.86, 90.00 |
Refinement procedure
Resolution | 38.890 - 1.700 |
R-factor | 0.174 |
Rwork | 0.174 |
R-free | 0.20400 |
Structure solution method | SIRAS |
RMSD bond length | 0.005 |
RMSD bond angle | 0.900 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.900 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.072 | 0.360 |
Number of reflections | 27251 | |
<I/σ(I)> | 28.4 | 4.64 |
Completeness [%] | 99.9 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 20% PEG 8000, 50mM potassium dihydrogenphosphate, 5% glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |