2D7J

Crystal Structure Analysis of Glutamine Amidotransferase from Pyrococcus horikoshii OT3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Collection date	2003-06-10
Wavelength(s)	0.9740
Spacegroup name	P 32 2 1
Unit cell lengths	64.941, 64.941, 116.408
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.920 - 1.890
R-factor	0.18584
Rwork	0.184
R-free	0.21334
Structure solution method	SAD
RMSD bond length	0.028
RMSD bond angle	2.129
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.890	1.890
Number of reflections	23560
Completeness [%]		100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	278	0.1M sodium acetate, 2M sodium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K