2D7F
Crystal structure of A lectin from canavalia gladiata seeds complexed with alpha-methyl-mannoside and alpha-aminobutyric acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-05-06 |
Detector | MARRESEARCH |
Wavelength(s) | 1.43 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 100.915, 115.754, 241.626 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.990 - 2.310 |
R-factor | 0.18206 |
Rwork | 0.180 |
R-free | 0.22683 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.028 |
RMSD bond angle | 2.363 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.988 | 2.410 |
High resolution limit [Å] | 2.309 | 2.310 |
Rmerge | 0.073 | 0.354 |
Number of reflections | 61808 | |
Completeness [%] | 99.1 | 94.5 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | ammonium sulphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |