2D7E

Crystal structure of N-terminal domain of PriA from E.coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-06
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	H 3 2
Unit cell lengths	111.570, 111.570, 260.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.500
Rwork	0.247
R-free	0.29230
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.485
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.550
High resolution limit [Å]	2.500	2.500
Rmerge	0.036
Number of reflections	20806
<I/σ(I)>	38.4	7.5
Completeness [%]	97.2	94.2
Redundancy	12.55	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	0.1M sodium citrate, 0.3M ammonium sulfate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	0.1M sodium citrate, 0.3M ammonium sulfate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K