2D7D
Structural insights into the cryptic DNA dependent ATP-ase activity of UvrB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.231, 98.215, 95.405 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.22961 |
| Rwork | 0.227 |
| R-free | 0.28485 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d9x |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.610 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.082 | 0.310 |
| Number of reflections | 39593 | |
| <I/σ(I)> | 5.8 | 2.4 |
| Completeness [%] | 96.1 | 92.9 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 289 | 18-20% (w/v) PEG 10000 0.1M Tris-Hcl, pH 8.5, Microbatch, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 10000 | ||
| 2 | 1 | 1 | Tris-Hcl | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | PEG 10000 | ||
| 5 | 1 | 2 | Tris-Hcl | ||
| 6 | 1 | 2 | H2O |
