2D6N

Crystal structure of mouse galectin-9 N-terminal CRD in complex with N-acetyllactosamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	56.689, 58.575, 92.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.450 - 2.000
R-factor	0.1741
Rwork	0.171
R-free	0.23090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a3k
RMSD bond length	0.014
RMSD bond angle	1.545
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.118	0.256
Number of reflections	20106
Completeness [%]	93.6	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	289	5% PEG6000, 0.1M citrate (pH5.0), VAPOR DIFFUSION, HANGING DROP, temperature 289K