2D6M
Crystal structure of mouse galectin-9 N-terminal CRD in complex with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9780 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.643, 58.472, 92.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.390 - 1.600 |
| R-factor | 0.18513 |
| Rwork | 0.183 |
| R-free | 0.23197 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a3k |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.438 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.088 | 0.345 |
| Number of reflections | 38497 | |
| Completeness [%] | 93.7 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 5% PEG6000, 0.1M citrate (pH5.0), VAPOR DIFFUSION, HANGING DROP, temperature 289K |
